Chaotropic buffer substitution:
-Guanidine isothiocyanate (GITC) with Guanidine HCl
G-HCl 5M in 20ml
October 4, 2010.
Bottles
|
Soil (g)
|
Z Buffer (ml)
|
1
|
15.028g
|
22.5ml
|
2
|
15.12g
|
22.5ml
|
3
|
15.13g
|
22.5ml
|
4
|
15.05g
|
22.5ml
|
5
|
15.07g
|
22.5ml
|
6
|
15.05g
|
22.5ml
|
**Freeze and Thaw**
October 9, 2010.
DNA Purification
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